A 1H-NMR study of the activity expressed by lactate dehydrogenase in the human erythrocyte
β Scribed by Kevin M. BRINDLE; Iain D. CAMPBELL; Robert J. SIMPSON
- Book ID
- 115124596
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 858 KB
- Volume
- 158
- Category
- Article
- ISSN
- 1432-1327
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## Abstract The N(CH~3~)~3~ resonance of ergothioneine in ^1^H spinβecho Fourier transform (SEFT) NMR spectra of red blood cells is usually a large singlet and it has been common practice to use this apparently unchanging resonance as an intensity reference. Recently, Reglinski __et al__. (Magn. Re
The lactate concentration was estimated from the spectra by comparison to signals from N-acetylaspartate, creatine + phosphocreatine, and water. The effects of Tz, phase modulation, and solvent suppression were taken into account. The estimated concentrations were compared to determinations performe