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6-Deoxy-D-glucose and D-xylose: Analogs for the study of D-glucose transport by mouse 3T3 cells

✍ Scribed by Antonio H. Romano; Nancy D. Connell

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
662 KB
Volume
111
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

6‐Deoxy‐D‐glucose and D‐xylose, structural homomorphs of D‐glucose that lack a 6‐hydroxyl group or a 6‐hydroxymethyl group, respectively, are transported efficiently by mouse 3T3 cells, with good affinity and high specificity for the D‐glucose transport system. Since these analogs lack the 6‐hydroxyl group, which is the site of phosphorylation of glucose by hexokinase, they are taken up and are recoverable from cells in an unchanged state. Thus, 6‐deoxy‐D‐glucose and D‐xylose offer advantages as transport substrates over 2‐deoxy‐D‐glucose, which is phosphorylated by intercellular hexokinases, and 3‐O‐methyl‐D‐glucose, which shows a lower specificity for the D‐glucose transport system.

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