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6-Azauracil inhibition of GTP biosynthesis inSaccharomyces cerevisiae

✍ Scribed by Françoise Exinger; François Lacroute

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
437 KB
Volume
22
Category
Article
ISSN
0172-8083

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✦ Synopsis

The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wildtype, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.

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Inhibition of thymidylate biosynthesis induces mitotic unequal sister chromatid recombination inSaccharomyces cerevisiae
✍ B. A. Kunz; G. R. Taylor; B. Konforti; B. W. Glickman; R. H. Haynes 📂 Article 📅 1984 🏛 Springer-Verlag 🌐 English ⚖ 699 KB

Inhibition of thymidylate biosynthesis has been found to induce deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of Saccharomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu -) and non-sectored (leu-