Inhibition of thymidylate biosynthesis induces mitotic unequal sister chromatid recombination inSaccharomyces cerevisiae
✍ Scribed by B. A. Kunz; G. R. Taylor; B. Konforti; B. W. Glickman; R. H. Haynes
- Publisher
- Springer-Verlag
- Year
- 1984
- Tongue
- English
- Weight
- 699 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
Inhibition of thymidylate biosynthesis has been found to induce deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of Saccharomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu -) and non-sectored (leu-) colonies. Hybridization patterns obtained by Southern blot analysis of DNA from the leu + and leu-sectors were consistent with the occurrence of unequal sister chromatid recombination. The induction of sectored colonies was prevented by the rad52-1 mutation but not by defects in RAD6. However, the formation of non-sectored leucolonies was induced by thymidylate depletion in both rad52-1 and rad6 strains.