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5212067 Quick assay for detection of salmonella


Book ID
123269435
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
65 KB
Volume
12
Category
Article
ISSN
0734-9750

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Molecular Beacons: A Real-Time Polymeras
โœ Wilfred Chen; Grisselle Martinez; Ashok Mulchandani ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 146 KB

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea