The antisense therapeutic strategy makes the assumption that sequence-specific hybridization of an oligonucleotide to its target can take place in living cells. The present work provides a new method for the detection of intracellular RNA molecules using in situ hybridization on living cells. The fi
✦ LIBER ✦
50 Specific detection of RNA molecules by fluorescentin situhybridization in living cells
✍ Scribed by S. Paillasson; M. Robert-Nicoud; X. Ronot
- Book ID
- 104773553
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 103 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0742-2091
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## Abstract By means of ^3^H thymidine‐labelled adenovirus 2 DNA, adenovirus‐specific DNA sequences have been localized in individual nuclei of adenovirus 2‐ or 12‐infected cells, and adenovirus‐specific RNA sequences have been detected in the permissive cells as well as in cells transformed by ade