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4916212 DNA-sequence encoding biosynthetic insulin precursors and process for preparing the insulin precursors and human insulin


Book ID
122923471
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
70 KB
Volume
8
Category
Article
ISSN
0734-9750

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The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor. A transformant with a high copy number of the gene integrated into the chromosome was obtained by the dot-blotting method. In high-density fermentation