4.7. Direct immune-fixation in Gc-subtyping after isoelectric focusing in agarose gels
โ Scribed by W. Martin
- Book ID
- 113369255
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 76 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0379-0738
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## Olof Vesterberg Sensitive silver staining of proteins after isoelectric focusing in agarose gels
In this paper a new method for immunological detection of small peptides after isoelectric focusing in thin (200 micron) polyacrylamide slab gels is presented. The peptides are immobilized immediately after focusing by pressing a sheet of glutaraldehyde-impregnated filter paper onto the gel. By this
A powerful method for group specific component (GC) subtyping with good resolution of GC bands by isoelectric focusing on ultrathin immobilized pH gradients is described. After separation, GC detection is achieved using a highly sensitive alkaline phosphatase conjugated enzyme-immuno system. The eff