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[3H]Thymidin-Einbau und DNA-Synthese beiFunaria hygrometrica

✍ Scribed by Bernd Knoop


Publisher
Springer-Verlag
Year
1974
Tongue
English
Weight
342 KB
Volume
121
Category
Article
ISSN
0032-0935

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✦ Synopsis


Incubation of caulonema filaments of the moss Funaria hygrometrica with [(3)H]methylthymidine followed by autoradiography fails to show the real amount of DNA-synthesis. Especially nuclei of steadily growing tip cells remain unlabelled, whereas basic cells become radioactive. This effect is not based on different permeabilities. Autoradiographic results of experiments with 5-fluordesoxyuridine and cycloheximide lead to the conclusion that there exists a marked thymidine-kinase gradient along the filament which depends on age and position of the cells.


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