[3H]Thymidin-Einbau und DNA-Synthese beiFunaria hygrometrica
✍ Scribed by Bernd Knoop
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 342 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Incubation of caulonema filaments of the moss Funaria hygrometrica with [(3)H]methylthymidine followed by autoradiography fails to show the real amount of DNA-synthesis. Especially nuclei of steadily growing tip cells remain unlabelled, whereas basic cells become radioactive. This effect is not based on different permeabilities. Autoradiographic results of experiments with 5-fluordesoxyuridine and cycloheximide lead to the conclusion that there exists a marked thymidine-kinase gradient along the filament which depends on age and position of the cells.
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