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3D Mapping of Individual Ion Channels on Living Cells

✍ Scribed by Gerhard J. Schütz; Jan Hesse; Günter Freudenthaler; Vassili Ph. Pastushenkoo; Hans-G?nter Knaus; Bernt Pragl; Hansgeorg Schindler

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
394 KB
Volume
1
Category
Article
ISSN
1438-5163

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High affinity binding of fluorescence labeled hongotoxin (HgTX 1 -Cy5) to the potassium channel K V 1.3 in T-lymphocyte cell membranes was utilized for imaging single ion channels optically, employing Single Dye Tracing (SDT). Binding sites were seen as single fluorescence peaks in cross-sections th