31P nuclear magnetic resonance study of acute renal dysfunction in rat kidney transplants

✍ Scribed by J. I. Shapiro; C. E. Haug; R. Weil III; L. Chan

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 349 KB
• Volume: 5
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910050405

✦ Synopsis

NMR spectroscopy was used to study renal allografts in rats subjected to allograft rejection, cyclosporine toxicity, ischemia, and ureteral obstruction. Parameters of relative peak areas and intracellular pH were accurately distinguished among the different causes of graft dysfunction. Ureteral obstruction was clearly identified by elevations in the phosphodiester/urine phosphate peak. Ischemia and rejection were. both associated with increases in inorganic phosphates and phosphomonesters and decreases in the j3-phosphate peak of adenosine triphosphate but were distinguishable from each other by differences in intra-cellularpH which was normal in rejected allografts (7.33 f 0.07, n = 3) but low in ischemic allografts (7.00 -C 0.05, n = 3, P i 0.05). Grafts insulted with cyclosporine toxicity were not distinguishable from normal allografts by any of the parameters studied. These data suggest that 31P NMR spectroscopy may have potential clinical application in differentiating among the causes of graft failure of human rend dlografts.