31P Nuclear Magnetic Resonance Analysis of Phospholipids in a Ternary Homogeneous System

✍ Scribed by M. Branca; N. Culeddu; M. Fruianu; M.V. Serra

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 144 KB
• Volume: 232
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.9968

✦ Synopsis

EDTA-K salt in water and, as described in Refs. 1 and We analyzed the changes in the shape and resonance 4-6, we obtained NMR spectra with sharp and wellpositions of the 31 P NMR signals of phospholipid mixresolved peaks. An aqueous phase and a chloroformic tures in a single-phase solvent containing different phase were formed by mixing a chloroform solution of quantities of chloroform, methanol, and water. We obphospholipids with a reagent containing methanol and tained extremely reproducible NMR signals when suf-EDTA-K salt in water. In the chloroformic phase, we ficient care was taken to control the desired solvent obtained a sharp and well-resolved NMR spectrum. composition. The solvent composition can be pre-This spectrum, however, was not always reproducible.

dicted using a ''tie line'' in a ternary diagram of chlorophosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin.