31P NMR Study of Acute Toxic Effects of Cadmium Chloride on Rat Liver

✍ Scribed by Hiroshi Yoshioka; Yuji Itai; Fumiyuki Mitsumori

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 552 KB
• Volume: 33
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910330609

✦ Synopsis

Abstract

In this study, acute effects of cadmium ions (Cd^2+^) on energy metabolism in rat livers were analyzed in vivo after intravenous administration using ^31^P NMR. Both inorganic phosphate (P~i~) and nucleotide triphosphate (NTP) peaks of in vivo Cdtreated livers gradually decreased over a 6‐h period. In the extract, NTP peaks in Cdtreated livers were lower, as in the in vivo experiments, but the P~i~ peak was significantly higher than the control. The apparent decrease in P~i~ in in vivo liver treated with Cd could be caused by the reduced visibility of P~i~ because of its uptake into mitochondria from cytoplasm, accompanied by the uncoupling of oxidative phosphorylation by Cd^2+^. These results indicated that total P~i~ in the hepatocytes increases after Cd administration. However, only 10% of P~i~ was visible in Cd‐treated livers in vivo, whereas 34% of P~i~ was visible in controls. Significant increases in phosphocholine and glycerophosphocholine were also observed in extracts of Cd‐treated livers.