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31P and 1H NMR spectroscopic studies of liver extracts of carbon tetrachloride-treated rats

✍ Scribed by Peta J. Harvey; Jill E. Gready; Haruyo M. Hickey; David G. Le Couteur; Allan J. McLean

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 85 KB
Volume: 12
Category: Article
ISSN: 0952-3480
DOI: 10.1002/(sici)1099-1492(199910)12:6<395::aid-nbm568>3.0.co;2-m

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✦ Synopsis

NMR spectroscopy was used to examine hepatic metabolism in cirrhosis with a particular focus on markers of functional cellular hypoxia. 31 P and 1 H NMR spectra were obtained from liver extracts from control rats and from rats with carbon tetrachloride-induced cirrhosis. A decrease of 34% in total phosphorus content was observed in cirrhotic rats, parallelling a reduction of 40% in hepatocyte mass as determined by morphometric analysis. Hypoxia appeared to be present in cirrhotic rats, as evidenced by increased inorganic phosphate levels, decreased ATP levels, decreased ATP:ADP ratios (1.72 AE 0.40 vs 2.48 AE 0.50, p 0.01), and increased inorganic phosphate:ATP ratios (2.77 AE 0.48 vs 1.62 AE 0.24, p 0.00001). When expressed as a percentage of the total phosphorus content, higher levels of phosphoethanolamine and lower levels of NAD and glycerophosphoethanolamine were detected in cirrhotic rats. Cirrhotic rats also had increased phosphomonoester:phosphodiester ratios (5.73 AE 2.88 vs 2.53 AE 0.52, p `0.01). These findings are indicative of extensive changes in cellular metabolism in the cirrhotic liver, with many findings attributable to the presence of intracellular hypoxia.

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