FTIR spectroscopic and HPLC chromatographic studies of carbon tetrachloride induced acute hepatitis in rats: Damage in liver phospholipid membrane
✍ Scribed by Seokjoo Yoon; Akio Kazusaka; Shoichi Fujita
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2000
- Tongue
- English
- Weight
- 141 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Carbon tetrachloride (CCl 4 ) induced rat hepatitis was studied by observing an FTIR spectrum of the liver microsomal or homogenate extract compared with those of model compounds. The microsomal extract from the liver of healthy control rats showed almost the same spectrum as a mixture of phosphatidylcholine and phosphatidylethanolamine (2:1 by weight). Intraperitoneal injection of CCl 4 decreased the absorption intensity due to the OCOH in the OCACOH at 3012 cm Ϫ1 in the microsomal extract, and it developed a new 1,2-diacylglycerol band at 1070 cm Ϫ1 in the homogenate extract. An HPLC study was added to assign the 1070 cm Ϫ1 band to 1,2-diacylglycerol. These findings were interpreted from the peroxidation of the microsomal membrane and the regenerative proliferation of the damaged cell.