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31P NMR spectroscopy of isolated perfused lungs

✍ Scribed by R. P. Pillai; P. C. Buescher; D. B. Pearse; J. T. Sylvester; G. L. Eichhorn

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
308 KB
Volume
3
Category
Article
ISSN
0740-3194

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✦ Synopsis

P NMR spectra of isolated blood-perfused pig lungs were obtained by degassing the lungs in vivo to remove field inhomogeneities caused by air-tissue interfaces. The spectra show the presence of ATP, phosphodiester, inorganic phosphate, and phosphomonoester, but no phosphocreatine. All the metabolites remained stable for more than 4 h when the lungs were perfused with oxygenated blood. Blood gas tensions, glucose concentration, pH, and temperature were controlled throughout the experiment. During anoxia or isch-

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