A 31P NMR study of preconditioned isolated perfused rat heart exposed to intermittent ischemia

✍ Scribed by Ram Sharoni; Abira Olivson; Mukul Chandra; Gideon Merin; Gideon Uretzky; Joseph B. Borman; Roland Chisin; Herzl Schwalb; Elisha Berman

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 618 KB
• Volume: 36
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910360112

✦ Synopsis

Abstract

Exposure to a short ischemic period (ischemic preconditioning, IP) will protect the heart from damage following a subsequent longer ischemic episode. The aim of the study was to test whether IP is cardioprotective in the setting of repeated ischemia‐reperfusion cycles. Thus, Langendorff‐perfused hearts, exposed to IP, were subjected to three consecutive ischemia‐reperfusion (10/15 min) cycles. Myocardial energetics, manifested by ^31^P NMR spectroscopy, was correlated with hemodynamics. ATP recovery was significantly higher for the IP group compared with control (P < 0.02) during reperfusions. However, there was no significant difference in ATP recovery during the three ischemic intervals. The supernormal recovery of phosphocreatine recorded during reperfusion was lower for the IP group (∼120%) compared with control (∼135%, P < 0.065). Better recovery of the left ventriculardeveloped pressure was noted during reperfusions for the IP group and became significant only during the last reperfusion (86% versus 68%, P < 0.025). In conclusion, the above results support prolonged IP cardioprotection.