31P NMR measurement of ATP synthesis rate in perfused intact rat hearts
โ Scribed by P. Kingsley-Hickman; E.Y. Sako; P.A. Andreone; J.A. St. Cyr; S. Michurski; J.E. Foker; A.H.L. From; M. Petein; K. Ugurbil
- Book ID
- 115918649
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 632 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0014-5793
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## Abstract The ability to measure ATP synthesis rates using ^31^PโNMR spectroscopy is demonstrated in the normal, ischemic, and postischemic myocardium __in vivo.__ Cardiopulmonary bypass (CBP) was employed to induce 20 min of global myocardial ischemia, and to conduct magnetization transfer measu
NMR spectroscopy was utilized to study phosphate metabolism of perfused ovaries. In luteinized ovaries, a loss in ATP of -30% with a concomitant acidification was reversed by 50 pMadenosine. The results suggest that in the ovaries, adenosine can serve to regulate ATP concentration during periodic h