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31P-NMR studies on membrane phospholipids in microsomes, rat liver slices and intact perfused rat liver

✍ Scribed by B. de Kruijff; A. Rietveld; P.R. Cullis

Book ID
115724787
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
820 KB
Volume
600
Category
Article
ISSN
0005-2736

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The phosphorus N M R spectra of isolated perfused rat liver displays a prominent inorganic phosphate peak at 2.76 f 0.05 ppm relative to liver glycerolphosphocholine at 0.49 ppm. From titration curves of phosphoruscontaining compounds this corresponds to a pH of 7.4. The spectra also display a shoul

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We examined the route of uptake of 2-aminoethylphosphonate (NEthPo) and of phenylphosphonate (PhePo; 10 mM each) in perfused liver by 31 P-NMR. Uptake of NEthPo was concentrative. The rate of uptake was reduced to 21 AE 2% (n = 3; all percentages refer to control rates) by substituting choline for N