✦ LIBER ✦

31P NMR assessment of orthotopic liver rejection in a rat model

✍ Scribed by John L. Bowers; Katsunori Kawano; Kenneth R. Metz; Kenichi Teramoto; Ann McCullough; Melvin E. Clouse

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 657 KB
Volume: 32
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910320203

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

^31^P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (P~i~), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and P~i~ on Day 7. High‐resolution ^31^P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (P~i~ + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

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