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296 ISOLATION, CHARACTERIZATION AND HEPATOCYTE DIFFERENTIATION OF HUMAN ADULT PROGENITOR CELLS FROM LIVER AND PANCREAS

✍ Scribed by Duret, C.; Iankova, I.; Ramos, J.; Gerbal-Chaloin, S.; Fabre, J.-M.; Wojtusciszyn, A.; Maurel, P.; Daujat-Chavanieu, M.

Book ID
123104594
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
57 KB
Volume
58
Category
Article
ISSN
0168-8278

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte