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25 - PCR-SSCP technique for the detection of mutation in the exons 5 and 6 of the p53 gene in breast cancer. Higher sensitivity than immunohistochemical technique

✍ Scribed by A. Dueñas; J.J Cruz; M.M Abad; R. GonzáleZ-Sarmient; C.A. Rodríguez; E. Fonseca; A. Gómez; G. Martín; P. Sánchez; R. Salazar


Book ID
117664287
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
266 KB
Volume
33
Category
Article
ISSN
0959-8049

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In primary human breast carcinomas mutat
✍ Giorgio R. Merlo; Amelia Bernardi; Francesca Diella; Tiziana Venesio; Alberto P. 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 French ⚖ 748 KB

## Abstract A series of 121 human breast tumors was screened for point mutations in exons 5 through 8 of the p53 gene, by SSCP analysis. On the same tumor samples, the S‐phase index (SPI) was determined by the incorporation of BUdR in fresh tissue. p53 mutations were observed in 29% of the cases. T