2,3-diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine

✍ Scribed by Peter J. Tarcha; Victor P. Chu; Dave Whittern

• Book ID: 102628699
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 249 KB
• Volume: 165
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90224-7

✦ Synopsis

NMR and mass spectroscopic evidence has been obtained which indicates that the product of the oxidation of o-phenylenediamine by hydrogen peroxide, uncatalyzed or catalyzed by horseradish peroxidase, is 2,3-diaminophenazine. These results settle disparate literature descriptions. The process is most likely free radical in nature starting with the abstraction of a labile amino hydrogen atom.