𝔖 Bobbio Scriptorium
✦   LIBER   ✦

20. Improved cryopreservation protocols for brain cell aggregates

✍ Scribed by *Rita Malpique; Friederike Ehrhart; Luísa Osório; Daniela Ferreira; Heiko Zimmermann; Paula M. Alves


Book ID
117981940
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
57 KB
Volume
59
Category
Article
ISSN
0011-2240

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Improved protocols for the isolation and
✍ Jan-Heiner Küpper; Marcus Müller 📂 Article 📅 1996 🏛 Springer 🌐 English ⚖ 582 KB

Stable transfection and cloning of cells often require physical separation of cell colonies. In order to conveniently isolate cell clones from petri dishes, we developed a protocol starting with a soft agar overlay of cells. This reduces the risk of cell diffusion between different colonies. Cells f