65. Cryopreservation of human embryonic stem cells: An improved protocol by programmed cooling
β Scribed by Peng-Fei Yang; Qi-Feng Wang; Tse-Chao Hua
- Book ID
- 116392498
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 41 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0011-2240
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Human embryonic stem cells (hESCs) represent an important resource for novel cell-based regenerative medical therapies. hESCs are known to differentiate into mature cells of defined lineages through the formation of embryoid bodies (EBs) which are amenable to suspension culture for several weeks. Ho
## Abstract In this study a three step culture system, 2Dβ3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Fiveβdayβold embryoid bodies were plated for chondrogenic induction for 27 days (