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2-Aminopurine induced mutations in T4 bacteriophage

✍ Scribed by Vigier, P. R. R. ;Marcovich, H.

Publisher
Springer
Year
1973
Tongue
English
Weight
455 KB
Volume
127
Category
Article
ISSN
0026-8925

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We have previously demonstrated that the sequence 5'TGGCAA 3' located at codons 32-33 of the rIIB gene of bacteriophage T4 is a hotspot for transition mutations (Nelson et al. 1981). Here I report the properties of the same TGGCAA sequence introduced into the gene at codons 11-12. The sequence is hi

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The survival of UV-irradiated lambda phages is increased when host bacteria are grown in the presence of the base analogue 2-aminopurine (2AP) before infection. This increase in survival, which we have called "2AP-reactivation" depends upon the concentration of 2AP and the time of exposure to 2AP. 2