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1α,25-Dihydroxyvitamin D3-induced changes in intracellular pH in osteoblast-like cells modulate gene expression

✍ Scribed by Louis G. Jenis; Jane B. Lian; Gary S. Stein; Dr. Daniel T. Baran

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
563 KB
Volume
53
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

1α,25‐Dihydroxyvitamin D~3~ exerts rapid nongenomic effects on rat osteoblast‐like cells independent of the classic nuclear receptor. These effects include changes in phospholipid metabolism and cell calcium. Intracellular calcium itself has been proposed to regulate intracellular pH in osteoblast cell lines. The purpose of this study was to determine the effect of 1α,25‐dihydroxyvitamin D~3~ on intracellular pH, the relationship of changes in calcium to changes in pH, and the role of pH changes in genomic activation. 1α,25‐Dihydroxyvitamin D~3~ increased intracellular pH within 10 min in rat osteoblast‐like cells, an effect that was inhibited by removal of extracellular sodium and by the biologically inactive epimer 1β,25‐dihydroxyvitamin D~3~. The hormone increased intracellular calcium in Quin 2 loaded cells in the presence and absence of extracellular sodium. The 1α,25‐dihydroxyvitamin D~3~‐induced increments in osteocalcin and osteopontin mRNA levels were abolished in sodium‐free medium. The results indicate that 1α,25‐dihydroxyvitamin D~3~‐induced increments in cellular calcium precede cell alkalinization and that these changes in intracellular pH may modulate steady‐state mRNA levels of genes induced by vitamin D.

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