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1H NMR study of the interaction of bacteriophage λ Cro protein with theOR3operator

✍ Scribed by K. D. Hahn; F. Buck; H. Rüterjans; B. K. Chernov; K. G. Skryabin; M. P. Kirpichnikov

Book ID
104688525
Publisher
Springer
Year
1985
Tongue
English
Weight
908 KB
Volume
12
Category
Article
ISSN
1432-1017

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✦ Synopsis

The 17 base pair operator OR3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage lambda, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exchangeable base protons and the H1' and the H2'-H2" sugar protons of the OR3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the OR3 operator - Cro repressor interaction.

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