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15 N Metabolic Labeling of Mammalian Tissue with Slow Protein Turnover

โœ Scribed by McClatchy, Daniel B.; Dong, Meng-Qiu; Wu, Christine C.; Venable, John D.; Yates, John R.


Book ID
121280628
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
182 KB
Volume
6
Category
Article
ISSN
1535-3893

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For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryp