133Ba cryptate labeled immunoglobulin G

✍ Scribed by William A. Pettit; Barbara K. Swailes

• Book ID: 102369217
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 379 KB
• Volume: 33
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580330409

✦ Synopsis

Abstract

A method for the attachment of cryptates containing radioactive metal ions to proteins has been developed. The aminocryptand, 4,7,13,16,21,24‐hexaoxa‐5,6‐(4′‐aminobenzo)1,10‐diazabicyclo[8.8.8]‐hexacosane, was complexed with divalent ^133^Ba and subsequently converted to the isothiocyanatocryptate. The bioconjugate with immunoglobulin G was formed in better than 40% radiochemical yield in a three step procedure. Alternatively, the radiolabeled bioconjugate could be formed by addition of ^133^Ba to immunoglobulin G that had been reacted with the isothiocyanate derivative of the cryptand. Stability studies indicated the radiolabeled bioconjugate has a half‐life of 6 h and 12 h in phosphate buffers of pH=7.4 and pH=9.2, respectively. Attempts to label the immunoglobulin with the corresponding ^142^Pr cryptate resulted in considerable radioactive colloid formation with no detectable bioconjugate produced.