1,2-Dihydro-1,2-dihydroxynaphthalene dehydrogenase containing recombinant strains: Preparation, isolation and characterisation of 1,2-dihydroxynaphthalenes and 1,2-naphthoquinones

✍ Scribed by Camilla Cavallotti; Fulvia Orsini; Guido Sello; Patrizia Di Gennaro; Enrica Galli; Giuseppina Bestetti

• Book ID: 104209301
• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 760 KB
• Volume: 55
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(99)00135-0

✦ Synopsis

1,2-Dihydroxynaphthalenes are produced by dehydrogenation of the corresponding 1,2dihydro-l,2-dihydroxynaphthalenes using an Escherichia coli recombinant strain containing the dihydrodiol naphthalene dehydrogenase gene cloned from Pseudomonasfluorescens N3, Conversions are led in carefully controlled conditions to minimise product polymerisation. A multistep procedure using a weakly basic resin permits isolation of good product amounts, solving the toxicity problem. Products are isolated and characterised as t-butyldimethylsilyl derivatives that are stable compounds. The transformation of the 1,2-dihydroxynaphthalenes into the corresponding 1,2-naphthoquinones is also reported.