025 An HPLC method for the determination of vitamin E (alpha tocopherol) in serum
✍ Scribed by Sabine Seitz; Rüdiger Kock; Helmut Greiling
- Book ID
- 112293003
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 204 KB
- Volume
- 343
- Category
- Article
- ISSN
- 1618-2650
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## Abstract A simple and rapid HPLC method requiring small volumes (250 μL) of human serum after C18 SPE sample preparation was developed using monolithic technology for simultaneous determination of all‐__trans‐__retinoic acid, 13‐__cis__‐retinoic acid, retinol, gamma‐ and alpha‐tocopherol. The mo
The method described here is essentially a modification of the method of Dilley and Crane (1) but has the advantages of greater speed and simplicity and gives higher recoveries. ## MATERIALS Benzene, petroleum ether (S&80), diethyl ether, absolute ethanol, and acetone were all analytical reagent