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Δ7-Sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles

✍ Scribed by Tania Husselstein; Hubert Schaller; Daniel Gachotte; Pierre Benveniste

Book ID
110232222
Publisher
Springer
Year
1999
Tongue
English
Weight
291 KB
Volume
39
Category
Article
ISSN
0167-4412

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✍ Daniel Gachotte; Tania Husselstein; Martin Bard; François Lacroute; Pierre Benve 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 518 KB

A yeast null mutant (__erg 3__) defective in __ERG 3__, the gene encoding the C‐5 sterol desaturase required for ergosterol synthesis was transformed with an __Arabidopsis thaliana__ cDNA library inserted in a yeast vector. Transformants (4×10^5^) were screened for cycloheximide (CH) resistance and