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β-Methylthio-aspartic acid: Identification of a novel posttranslational modification in ribosomal protein S12 from escherichia coli

✍ Scribed by Jeffrey A. Kowalak; Kenneth A. Walsh


Publisher
Cold Spring Harbor Laboratory Press
Year
1996
Tongue
English
Weight
639 KB
Volume
5
Category
Article
ISSN
0961-8368

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✦ Synopsis


Abstract

Utilizing microscale chemical derivatization reactions and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry, we have identified a novel posttranslational modification of aspartic acid, β‐methylthio‐aspartic acid. The modified residue is located at position 88 in ribosomal protein S12 from Escherichia coli, a phylogenetically conserved protein that has been implicated in maintaining translational accuracy of the ribosome.