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β-Galactosidase Activity in Transfected Ltk- Cells is Differentially Regulated in Monolayer and in Spheroid Cultures

✍ Scribed by Irene Klünder; Dieter F. Hülser


Book ID
115601502
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
896 KB
Volume
207
Category
Article
ISSN
0014-4827

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The b-galactosidase activity encoded by the lacZ gene of Escherichia coli is widely used to monitor successful expression of transfected genes. Fluorogenic substrates allow detection of enzyme activity in viable cells, which, subsequently, can be selected for further study on the basis of fluorescen