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β-Endorphin biotransformation in brain: Formation of γ-endorphin by a synaptosomal plasma membrane associated endopeptidase distinct from cathepsin D

✍ Scribed by J.P.H. Burbach; J.G. Loeber; J. Verhoef; E.R. de Kloet

Book ID
118314434
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
434 KB
Volume
92
Category
Article
ISSN
0006-291X

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Quantitation of the endopeptidase activi
Quantitation of the endopeptidase activity generating γ-endorphin from β-endorphin in rat brain synaptic membranes by a radiometric assay
✍ Jos L.M. Lebouille; J.Peter H. Burbach; E.Ronald de Kloet 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 768 KB

gamma-Endorphin is a naturally occurring biologically active peptide that is produced by an endopeptidase activity cleaving its precursor beta-endorphin. This enzyme was termed gamma-endorphin generating enzyme (gamma-EGE). In order to quantitate gamma-EGE activity by means of a simple and sensitive