α1 / α2 domains of H-2Dd, but not H-2Ld, induce “missing self” reactivity in vivo – No effect of H-2Ld on protection against NK cells expressing the inhibitory receptor Ly49G2
✍ Scribed by Maria H. Johansson; Elin Höglund; Mary C. Nakamura; James C. Ryan
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 141 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Introduction of the MHC class I transgene H-2D d on C57BL/6 (B6) background conveys NK cell-mediated "missing self" reactivity against transgene-negative cells, and down-regulates expression of the inhibitory receptors Ly49A and Ly49G2 in NK cells. We here present an analysis of transgenic mice expressing chimeric H-2D d /L d MHC class I transgenes, and show that the § 1/ § 2 domains of H-2D d were necessary and sufficient to induce "missing self" recognition and to down-modulate Ly49A and Ly49G2 receptors. In contrast, transgenes containing the § 1/ § 2 domains of H-2L d induced none of these changes, suggesting that not all MHC class I alleles in a host necessarily take part in NK cell education. The lack of effect of the § 1/ § 2 domains of H-2L d on NK cell specificity was surprising, considering that both H-2L d and H-2D d have been reported to interact with Ly49G2. Therefore, the role of H-2L d for protection against NK cells expressing Ly49G2 was re-investigated in a transfection system. In contradiction to earlier reports, we show that H-2D d , but not H-2L d , abolished killing by sorted Ly49G2 + NK cells, indicating that H-2L d does not inhibit NK cells via the Ly49G2 receptor.