α-2,3-sialyltransferase type 3N and α-1,3-fucosyltransferase type VII are related to sialyl Lewisx synthesis and patient survival from lung carcinoma
✍ Scribed by Jun-ichi Ogawa; Hiroshi Inoue; Shirosaku Koide
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 199 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Background:
Biosynthesis of sialyl lewis(x) (sle(x)) requires a sialyltransferase for alpha-2,3-sialylation and a fucosyltransferase for alpha-1,3-fucosylation. to date, five human alpha-1,3-fucosyltransferase (fuc-t) genes and five human alpha-2,3-sialyltransferase (st) genes have been cloned. however, it is not known which enzyme is mainly responsible for sle(x) synthesis.
Methods:
Three hundred thirteen patients with nonsmall cell lung carcinoma who had a curative tumor resection were the subjects of this study. using tumor tissues fixed in formaldehyde, amplification of genomic dna of fuc-t and st was performed by pcr and correlated with sle(x) staining and patient prognosis.
Results:
The frequency of strong st3n and fuc-tvii amplification was significantly higher than that of stz, st4, fuc-tiii, fuc-tv, and fuc-tvi amplification (p < 0.01). the frequency of sle(x) staining was similar to st3n and fuc-tvii amplification. survival of the patients whose tumors had strong amplification of both st3n and fuc-tvii was significantly shorter than that of patients whose tumors had no amplification of either gene (p < 0.01). in a multivariate analysis of survival, fuc-tvii remained a statistically significant prognostic factor.
Conclusions:
In lung carcinoma, st3n and fuc-tvii may both be related to sle(x) synthesis, and fuc-tvii is a more important indicator of poor prognosis.