Über Umwandlungen von Progesteron und 16α-Hydroxy-progesteron durch Enzyme tierischer Gewebe. Über Steroide, 157. Mitteilung

✍ Scribed by A. Wettstein; R. Neher; H. J. Urech

• Publisher: John Wiley and Sons
• Year: 1959
• Tongue: German
• Weight: 992 KB
• Volume: 42
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19590420337

✦ Synopsis

Abstract

Radioactive 16α‐hydroxyprogesterone (V) on incubation with homogenates or slices of rat liver, using in the first instance TPNH or DPNH as hydrogen‐carrier, is converted into 3β,16α‐dihydroxy‐allopregnan‐20‐one(I), 3α, 16α‐dihydroxy‐pregnan‐ 20‐one (11) and 3α, 16α‐dihydroxy‐allopregnan‐20‐one(111) in maximum yields of 11.4, 15.9, and 39.3%, respectively. This shows that V may be a precursor of the genuine, reduced compounds 1‐111.

Radioactive progesterone is converted into the same three 16α‐hydroxysteroids by rat liver homogenate in yields of only 0.94, 0.86, and 0.72%, respectively.

With bovine adrenal homogenate an extremely low conversion of progesterone into 16α‐hydroxyprogesterone (0.096yo)and substance I (0.044%) is observed.

Crystalline 16α‐hydroxyprogesterone can be isolated from hog adrenals in a yield of approx. 150 mg per ton of tissue.