Über die Dünnschichtchromatographie von Lipiden 2. Mitteilung Untersuchungen von Gehirngewebe aus der weissen Substanz Multiple-Sklerose-Kranker

✍ Scribed by C. G. Honegger

• Publisher: John Wiley and Sons
• Year: 1962
• Tongue: German
• Weight: 616 KB
• Volume: 45
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19620450639

✦ Synopsis

The authors describe the incorporation of radioactive aminoacids into microsomes from Drosophila melafiogaster (P.upae). Enzymatic incorporation can only be shown after inhibition of a very active tyrosinase, which is present in the micro-soma1 fraction, by phenylthiourea.

The system has the following properties: (1) It requires ATP, Mg++, an ATP regenerating system, but not GTP. (2) Sucrose-washed microsomes with and without addition of pH 5 enzymes incorporate to the same extent. (3) Microsomes treated with deoxycholate or 0 , 0 5 ~ KC1 incorporate less than the untreated ones, the former being nevertheless stimulated by addition of pH 5 enzymes or the pH 5 precipitate of the KC1 extract. (4) Rat liver microsomes or pH 5 enzymes can be exchanged with the corresponding fraction from Drosophila.