Zur DNS-Synthesezeit in der menschlichen Epidermis

✍ Scribed by E. Christophers; E. Schaumlöffel

• Publisher: Springer-Verlag
• Year: 1967
• Tongue: English
• Weight: 793 KB
• Volume: 228
• Category: Article
• ISSN: 0340-3696
• DOI: 10.1007/bf00499330

✦ Synopsis

1. DNA-synthesizing time of the human epidermis has been determined in vitro by a double labelling autoradiographie technique using aH-thymidine. The DNA-synthesizing time is 5.9 hours at 37~ Elevated temperatures seem to shorten this period.

2. In control studies the increasing labelling index after prolonged incubation with 8H-thymidine at 37~ has been evaluated and the increase of labelled cells per hour determined. In computing these data, the length of the DNA-synthesizing time was found to be in accordance with the previously found values.

3. From this the generation time of the basal cell layer has been calculated to be about 6--7 days. The need for exact knowledge on the length of the different cell cycle phases is emphasized.