Zum metabolismus von phenylharnstoffen : I. Dünnschichtchromatographische trennung und nachweis von aliphatischen hydroxylaminen neben niederen aminen
✍ Scribed by Ingolf Schuphan; Friedbernd Geike
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 822 KB
- Volume
- 84
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Metabolism of phenylureas. I. Thin-layer chromatograplric separation and identijication of aliphatic hydroxylamines in the presence of aliphatic amines
Methyl-substituted hydroxylamines, which can be formed as metabolites from various pesticides, were easily separated in the presence of alkylamines by thin-layer chromatography (TLC). The volatile hydroxylamines were steam-distilled from crop samples, concentrated in diluted hydrochloric acid and then chromatographed as hydrochlorides in dioxane-ethanol-I N WC1 (3:3: 1) on cellulose layers. To ensure the identity of the hydroxylamines, they were converted to 2&dinitrophenyl and 4-chlorophenylcarbamoyl derivatives. These compounds could be separated by thinlayer chromatography on silica gel using several solvent systems. The detection limits were in the range of I pg for the hydrochlorides up to 0.1 pg for the 2,4-dinitrophenyl and 4-chlorophenylcarbamoyl derivatives, EINLEITUNG