Zn and Cu alteration in connection with astrocyte metallothionein I/II overexpression in the mouse brain upon physical stress
✍ Scribed by Mariano Beltramini; Cristina Di Pisa; Pamela Zambenedetti; Werner Wittkowski; Eugenio Mocchegiani; Massimo Musicco; Paolo Zatta
- Book ID
- 102224160
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 426 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0894-1491
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The distribution of metallothioneins I/II in the mouse brain and their specific area distribution upon physical stress were studied. To induce physical stress, groups of mice were subjected to total darkness for different periods (2 weeks, 1 month, and 2 months). The concentration of metallothioneins, evaluated by immunohistochemistry, as well as area‐specific protein expression, were found in the following quantitative order: corpus striatum, cerebellum, mesencephalon, hippocampus with fornix, parts of thalamus, and pons. All other brain areas were marginally affected, or even unaffected, in terms of immunopositive metallothionein reaction. Metallothionein I/II expression was compared with the immunopositivity of glial fibrillary acidic protein (GFAP). It is noteworthy that metallothioneins and GFAP are expressed in different types of astrocytes. © 2004 Wiley‐Liss, Inc.