Zebrafish tenascin-W, a new member of the tenascin family

✍ Scribed by Weber, Philipp ;Montag, Dirk ;Schachner, Melitta ;Bernhardt, Robert R.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 408 KB
• Volume: 35
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199804)35:1<1::aid-neu1>3.0.co;2-9

✦ Synopsis

A cDNA clone encoding tenascinpression by sclerotomal and neural crest cells contin-W, a novel member of the tenascin family, was isolated ued to be observed while expression in the somitic from a 20-to 28-h postfertilization (hpf) zebrafish mesoderm was decreased. In juvenile fish, tn-w was cDNA library on the basis of the conserved epidermal expressed weakly by cells in the myosepta and, more growth factor-like domains represented in all tenascin strongly, by presumably nonneuronal cells in the dormolecules. An open reading frame of 2796 base pairs sal root ganglia. In these tissues and at the same develencodes a mature protein consisting of heptad repeats, opmental stages, the expression of tn-w partially overa cysteine-rich amino terminal region, 3.5 epidermal lapped with the distribution of tn-c mRNA. In addigrowth factor-like repeats, five fibronectin type III tion, tn-c was expressed in the central nervous system homologous repeats, and a domain homologous to fi-(CNS) and in the axial mesoderm, neither of which brinogen. These domains are the typical modular eleexpressed tn-w at any of the age stages examined. The ments of molecules of the tenascin family. Sequence expression pattern of tn-w suggests an involvement in comparison demonstrated that TN-W shares homoloneural crest and sclerotome cell migration and in the gies with the members of the tenascin family but is formation of the skeleton. Similar and possibly overnot a species homolog of any identified tenascin. The lapping functions could also be performed by tn-c, expression pattern of tn-w was analyzed by in situ which appears to have additional functions during the hybridization in 1-day-old embryos, in 3-day-old lardevelopment of the CNS. ᭧ 1998 John Wiley & Sons, Inc. vae, and in juvenile zebrafish. At 24-25 hpf, tn-w