Yolk proteins in the house cricket,Acheta domesticus: Identification, characterization, and effect of ovariectomy upon their synthesis

✍ Scribed by Bradley, James T. ;Edwards, John S.

• Book ID: 102893491
• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 749 KB
• Volume: 204
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402040211

✦ Synopsis

Abstract

Four female‐specific yolk proteins were identified in the hemolymph of adult, ovulating Acheta domesticus through the use of SDS‐polyacrylamide gel electrophoresis. Molecular weights were estimated at 130,000; 97,000; 49,000; and 47,000 daltons, and all four proteins stained PAS‐positive. None of the yolk proteins were detectable in the blood of males or immature females.

The rapid appearance and accumulation of yolk proteins in the blood two to five days after adult ecdysis involves de novo synthesis of the proteins and is unaffected by ovariectomy or hemiovariectomy performed six to eight days prior to ecdysis. Although ovariectomy resulted in the accumulation of yolk proteins in the blood, a comparison of the specific activities of hemolymph proteins in ovariectomized, hemiovariectomized, and sham operated animals following ^3^H‐leucine injection suggest that the absence of ovarian tissue may lead to a reduction in the net rate of yolk protein synthesis.