Isolation And Identification Of Yeasts From Natural Habitats / John F. Spencer, Dorothy M. Spencer -- Maintenance And Culture Of Yeasts / John F. Spencer, Dorothy M. Spencer -- Mutagenesis In Yeast / John F. Spencer, Dorothy M. Spencer -- Rare-mating And Cytoduction In Saccharomyces Cerevisiae / John F. Spencer, Dorothy M. Spencer -- Protoplast Fusion In Saccharomyces Cerevisiae / Brendan P. Curran, Virginia C. Bugeja -- Meiotic Analysis / John F. Spencer, Dorothy M. Spencer -- Ascus Dissection / Carl Saunders-singer -- Karyotyping Of Yeast Strains By Pulsed-field Gel Electrophoresis / John R. Johnston -- Chromosomal Localization Of Genes Through Pulsed-field Gel Electrophoresis Techniques / Graham R. Bignell, Ivor H. Evans -- Isolation, Purification, And Analysis Of Nuclear Dna In Yeast Taxonomy / Ann Vaughan-martini, Alessandro Martini -- Isolation Of Yeast Dna / Peter Piper -- Isolation Of Mitochondrial Dna / Graham R. Bignell, Angela R. Miller, Ivor H. Evans --^ Isolation Of Yeast Plasma Membranes / Barry Panaretou, Peter Piper -- The Extraction And Analysis Of Sterols From Yeast / Michael A. Quail, Steven L. Kelly -- Peroxisome Isolation / Ben Distel, Inge Leij, Wilko Kos -- Transformation Of Lithium-treated Yeast Cells And The Selection Of Auxotrophic And Dominant Markers / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Biolistic Transformation Of Yeasts / Stephen A. Johnston, Michael J. Devit -- Genomic Yeast Dna Clone Banks: Construction And Gene Isolation / Graham R. Bignell, Ivor H. Evans -- Yeast Colony Hybridization / Matthew John Kleinman -- Determination Of Plasmid Copy Number In Yeast / Bernadette E. Jordan, Robert C. Mount, Christopher Hadfield -- Determination Of Chromosome Ploidy In Yeast / Christopher Hadfield, Bernadette E. Jordan, Robert C. Mount -- Chromosome Engineering In Yeast With A Site-specific Recombination System From A Heterologous Yeast Plasmid / Yasuji Oshima, Hiroyuki Araki, Hiroaki Matsuzaki --^ Insertional Mutagenesis By Ty Elements In Saccharomyces Cerevisiae / David J. Garfinkel -- Reporter Gene Systems For Assaying Gene Expression In Yeast / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Preparation And Use Of Yeast Cell-free Translation Lysate / Alan D. Hartley, Manuel A. Santos, David R. Colthurst, Michael F. Tuite -- Electrophoretic Analysis Of Yeast Proteins / Christopher M. Grant, Ian T. Fitch, Michael F. Tuite -- Preparation Of Total Rna / Alistair J. Brown -- Mrna Abundance And Half-life Measurements / Alistair J. Brown, Francis A. Sagliocco -- Polysome Analysis / Francis A. Sagliocco, Paul A. Moore, Alistair J. Brown -- Induction Of Heat Shock Proteins And Thermotolerance / Peter Piper -- Rhodamine B Assay For Estimating Activity Of Killer Toxins Permeabilizing Cytoplasmic Membranes / Vladimír Vondrejs, Zdena Palková --^ Nystatin-rhodamine B Assay For Estimating Activity Of Killer Toxin From Kluyveromyces Lactis / Zdena Palková, Vladimír Vondrejs -- Application Of Killer Toxins In Stepwise Selection Of Hybrids And Cybrids Obtained By Induced Protoplast Fusion / Vladimír Vondrejs, Zdena Palková, Zuzana Zemanová -- Killer Plaque Technique For Selecting Hybrids And Cybrids Obtained By Induced Protoplast Fusion / Zdena Palková, Vladimír Vondrejs -- Genotoxicity Testing In Schizosaccharomyces Pombe / Pamela Mcathey -- Purification And Quantification Of Saccharomyces Cerevisiae Cytochrome P450 / Ian Stansfield, Steven L. Kelly -- Calorimetry Of Whole Yeast Cells / Linda J. Ashby, Anthony E. Beezer -- Light Microscopy Methods / Eva Streiblová, Jiří Hašek -- Fluorescence Microscopy Methods / Jiří Hašek, Eva Streiblová -- Immunoelectron Microscopy / Ever-jan Tuinen. Edited By Ivor H. Evans. Includes Bibliographical References And Index.