Yeast Gene Analysis

✍ Scribed by Alistair J.P. Brown and Mick Tuite (Eds.)

• Publisher: Academic Press
• Year: 1998
• Tongue: English
• Leaves: 494
• Series: Methods in Microbiology 26
• Edition: 1st
• Category: Library

✦ Synopsis

Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Focuses on the methods most useful for the functional analysis of yeast genes
Allows researcher to identify which strategy to use without having to wade through numerous recipes
Includes techniques for mutagenesis, transcript analysis, transposon tagging, and use of reporter genes
Describes the use of tools for studying post-translational modifications in yeast
Includes useful appendices with handy basic yeast recipes and WWW addresses

✦ Table of Contents

Content:
Series Advisors
Page ii

Edited by
Page iii

Copyright page
Page iv

Contributors
Pages vii-x

Preface
Pages xi-xiii
Mick F Tuite, Alistair J.P. Brown

1 Introduction to Functional Analysis of the Yeast Genome Original Research Article
Pages 1-13
Stephen G. Oliver

2 Whole Chromosome Analysis Original Research Article
Pages 15-31
Edward J. Louis

3 The Bioinformatics of the Yeast Genome Original Research Article
Pages 33-51
Hans-Werner Mewes, Dmitrij Frishman, Alfred Zollner, Klaus Heumann

4 Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG Method Original Research Article
Pages 53-66
R. Daniel Gietz, Robin A. Woods

5 PCR-Based Gene Targeting in Saccharomyces cerevisiae Original Research Article
Pages 67-81
Achim Wach, Arndt Brachat, Corinne Rebischung, Sabine Steiner, Karine Pokorni, Stephan te Heesen, Peter Philippsen

6 Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles Original Research Article
Pages 83-99
Michael J.R. Stark

7 Ty Mutagenesis Original Research Article
Pages 101-117
David J. Garfinkel, M. Joan Curcio, Victoria Smith

8 Transcript Analysis Original Research Article
Pages 119-139
Alistair J.P. Brown, L. Michael Furness, David Bailey

9 Reporter Genes and their Use in Studying Yeast Gene Expression Original Research Article
Pages 141-146,146a,147-159
Nadejda Koloteva, John M.X. Hughes, John E.G. McCarthy

10 Transposon Tagging I: A Novel System for Monitoring Protein Production, Function and Localization Original Research Article
Pages 161-179
Petra Ross-Macdonald, Amy Sheehan, Carl Friddle, G. Shirleen Roeder, Michael Snyder

11 Transposon Tagging II: Exploration of Gene Function and Regulatory Networks in Yeast with the Mini-Mu Transposon Original Research Article
Pages 181-200
Monique Bolotin-Fukuhara

12 Immunological Approaches to the Study of Protein Localization in Yeast Original Research Article
Pages 201-221
Iain M. Hagan, Colin J. Stirling

13 Posttranslational Modifications of Secretory Proteins Original Research Article
Pages 223-238
F.M. Klis, A.F.J. Ram, R.C. Montijn, J.C. Kapteyn, L.H.P. Caro, J.H. Vossen, M.A.A. Van Berkel, S.S.C. Brekelmans, H. Van den Ende

14 Studying Posttranslational Modifications in Yeast: Intracellular Proteins Original Research Article
Pages 239-254
Michael J.R. Stark

15 Two-Hybrid Analysis of Protein-Protein Interactions in Yeast Original Research Article
Pages 255-268
John Rosamond

16 “Smart” Genetic Screens Original Research Article
Pages 269-295
Jesús De la Cruz, Marie-Claire Daugeron, Patrick Linder

17 Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes Original Research Article
Pages 297-336
Bas Teusink, Frank Baganz, Hans V. Westerhoff, Stephen G. Oliver

18 Identifying Stress Genes Original Research Article
Pages 337-349
Willem H. Mager, Kick Maurer, Peter W. Piper

19 Identifying Genes Encoding Components of the Protein Synthesis Machinery of the Yeast Saccharomyces cerevisiae Original Research Article
Pages 351-373
Mick F. Tuite, Ian Stansfield, Rudi J. Planta

20 MAP Kinase-Mediated Signal Transduction Pathways Original Research Article
Pages 375-393
María Molina, Humberto Martín, Miguel Sánchez, César Nombela

21 Analysis of the Candida albicans Genome Original Research Article
Pages 395-415
P.T. Magee

22 Applications of Yeast in the Discovery of Human Gene Function Original Research Article
Pages 417-430
Paul A. Moore

23 Yeast Mutant and Plasmid Collections Original Research Article
Pages 431-449
Karl-Dieter Entian, Peter Kötter

24 Yeast Gene Analysis in the Next Millennium Original Research Article
Pages 451-461
Alistair J.P. Brown, Mick F. Tuite

Appendix I: The Ribosomal Protein Genes of Saccharomyces cerevisiae Original Research Article
Pages 463-470
Rudi J. Planta, Ian Stansfield, Mick F. Tuite

Appendix II: Yeast Growth Original Research Article
Pages 471-477
Alistair J.P. Brown, Mick F. Tuite

Appendix III: Useful World Wide Web Addresses for Yeast Researchers Original Research Article
Pages 479-484
Alistair J.P. Brown

Appendix IV: Nomenclature for Yeast Genes and Proteins Original Research Article
Pages 485-489
Mick F. Tuite

Index
Pages 491-502