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Y-derived sequence detected in minute chromosomes by polymerase chain reaction and in situ hybridization

✍ Scribed by Masato Tsukahara; Shinya Matsuura; Fumio Kishi; Lynda K. Fisher; A. Dean Stock; Yoshinori Izumikawa; Kenji Naritomi; Tadashi Kajii


Book ID
115090297
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
408 KB
Volume
42
Category
Article
ISSN
0009-9163

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A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t