X-ray spectral studies of Ca2+-binding protein centers upon isomorphous replacements

Ca2+-Binding domain VI of rat calpain is

Ca2+-Binding domain VI of rat calpain is a homodimer in solution: Hydrodynamic, crystallization and preliminary X-ray diffraction studies

✍ Helen Blanchard; Yunge Li; Miroslaw Cygler; Cyril M. Kay; J. Simon C. Arthur; Pe 📂 Article 📅 2008 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 313 KB

## Abstract Abstract: The 21‐kDa calcium‐binding domain (VI) of the small subunit of rat calpain II has been expressed in __Escherichia coli__, purified, and crystallized. Two orthorhombic crystal forms have been obtained: space group P2~1~2~1~2~1~ with __a__ = 50.3, __b__ = 56.5, __c__ = 141.3 Å;