X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I
✍ Scribed by Ronald Swanstrom; Peter R. Shank
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 533 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Detection of both y-ray (lzSI) and high energy /3 (y"P) particle-emitting radioisotopes by autoradiography is significantly enhanced by the use of X-ray intensifying screens. We have compared five commercially available intensifying screens (containing different inorganic phosphors) in combination with a number of films and exposure conditions. One calcium tungstate (DuPont, Cronex Lighting Plus intensifying screen produces an 8-to lo-fold enhancement in the detection of 32P and a 30-to 40-fold enhancement in the detection of rz51 when an autoradiogram is made with Kodak RP ROYAL X-OMAT film at -70°C. With one intensifying screen, 10 dpm of 3zP and 50 dpm of izSI in an area of 10 mm2 are readily detectable in a 20-hr exposure.